Frequency of Extended-spectrum Beta-Lactamases-Producing Escherichia coli Among Out- and In-patients in Rafsanjan City, Iran

Background: The frequency of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains among clinical isolates has been steadily increasing, leading to limited treatment options. Objectives: This study aimed assess the antibiotic sensitivity E. and ESBL both out-patients in-patients. Methods: A total 390 were received at Ali-Ebn-e-Abitaleb Hospital laboratory in Rafsanjan. antibiogram, as well phenotypic genotypic detection isolates, conducted using Kirby-Bauer, combination disk confirmatory, polymerase chain reaction tests, respectively. Results: Of all 45.6% exhibited production. Among these 46.1% obtained from hospital wards, while 42.5% outpatients. Meropenem imipenem displayed sensitivities 97.2% 93.3%, respectively, whereas amikacin nitrofurantoin showed 89.7% 85.6%, for isolates. Notably, ceftriaxone, cefotaxime, cefixime, ampicillin, co-trimoxazole, nalidixic acid demonstrated high resistance rates, surpassing 50%. ESBL-producing more frequently observed blood samples (65%) wounds (60%) compared other tested Approximately 8.6% carried a single type gene, 38.5% multiple genes. Conclusions: data indicate prevalence in-patients, with some them acquiring two or three types enzymes. As result, their ability hydrolyze antibiotics increased, higher occurrence samples.